miR-148b-5p regulates hypercalciuria and calcium-containing nephrolithiasis

• DOI: 10.1007/s00018-024-05408-8
• Publication Date: 2024-08-25T06:01:43Z
• Authors: Wei Zhu; Zhen Zhou; Chengjie Wu; Zhicong Huang; Ruiyue Zhao; Xinlu Wang; Lianmin Luo; Yang Liu; Wen Zhong; Zhijian Zhao; Guoyao Ai; Jian Zhong; Shusheng Liu; Weijie Liu; Xuliang Pang; Yin Sun; Guohua Zeng
• Keywords: Urinary calcium

Calcium-containing stones represent the most common form of kidney calculi, frequently linked to idiopathic hypercalciuria, though their precise pathogenesis remains elusive. This research aimed elucidate molecular mechanisms involved by employing urinary exosomal microRNAs as proxies for renal tissue analysis. Elevated miR-148b-5p levels were observed in exosomes derived from patients with stones. Systemic administration rat models resulted heightened calcium excretion, whereas its inhibition reduced stone formation. RNA immunoprecipitation combined deep sequencing identified a suppressor calcitonin receptor (Calcr) expression, thereby promoting excretion and Mice deficient Calcr distal epithelial cells demonstrated elevated calcification. Mechanistically, regulated through circRNA-83536/miR-24-3p signaling pathway. Human samples corroborated these results. In summary, regulates formation calcium-containing via circRNA-83536/miR-24-3p/Calcr axis, presenting potential target novel therapeutic interventions prevent nephrolithiasis.