Direct regulation of Per2 by Roraa: insights into circadian and metabolic interplay in zebrafish

• DOI: 10.1007/s00018-025-05696-8
• Publication Date: 2025-05-06T07:45:30Z
• Authors: Miao Yang; Yan Liu; Zhilin Zhong; Yue Ou; Mingyong Wang; Yingbin Zhong; Chao Liu
• Keywords: PER2

Circadian rhythms are fundamental for regulating physiological processes in organisms, with disruptions often linked to metabolic disorders. This study investigated the role of roraa gene zebrafish, particularly its influence on circadian and regulation. Using quantitative PCR situ hybridization, we confirmed rhythmic expression explored oscillatory mechanisms. The construction knockout mutants revealed that absence disrupts clock function, as evidenced by reduced core genes altered behavioral rhythms, while transgenic zebrafish lines which overexpress just have opposite results. Additionally, demonstrated Roraa directly regulates per2 through RORE element promoter. Furthermore, transcriptome analysis indicated metabolism related genes, especially lipid were obviously changed roraa-/- compare WT. Our findings underscore critical coordinating processes, providing insights into potential therapeutic targets addressing disorders disruption.