Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice

• DOI: 10.1007/s00068-021-01621-3
• Publication Date: 2021-04-04T13:02:20Z
• Authors: Katharina Sommer; Heike Jakob; Tobias Kisch; Dirk Henrich; Ingo Marzi; Johannes Frank; Anna L. Sander
• Keywords: Systemic administration; CD31; Homing (biology); Endothelial progenitor cell

Stem cell transplantation is one of the most promising strategies to improve healing in chronic wounds as systemic administration endothelial progenitor cells (EPC) enhances by promoting neovascularization and homing though a high amount needed. In following study, we analysed whether local application can reduce number EPC needed achieving same beneficial effect on wound healing.Wound after or treatment with was monitored vivo creating standardized dorsum hairless mice measuring closure every second day. Systemic group received 2 × 106 i.v. locally treated 105 EPC, injected. As control PBS injection performed way. Expression CD31, VEGF, CD90 and, SDF-1α immunohistochemically for evaluation neovascularisation amelioration homing.Local (7.1 ± 0.45 SD) well (6.1 0.23 led significant acceleration compared controls (PBS local: 9.7 0.5 SD, 10.9 0.38 SD). enhanced CD31 expression day 6 wounding 9 comparison control. VEGF not significantly affected. resulted all days investigated.Local healing. Moreover, effects are obtained tenfold decrease when applied locally. Thus, might be more convenient way enhance limited.