Immunomodulatory and cardio-protective effects of differentially originated multipotent mesenchymal stroma cells during polymicrobial sepsis in mice

• DOI: 10.1007/s00068-025-02862-2
• Publication Date: 2025-04-20T11:42:45Z
• Authors: Anna Kanewska; Ina Lackner; Anne Friedrich; Martina Winkelmann; Markus Rojewski; Birte Weber; Jochen Preßmar; Mario Perl; Hubert Schrezenmeier; Miriam Kalbitz

Abstract Purpose Sepsis is a life-threatening condition with cardiac complications being an independent predictor of poor outcome. Although their mechanisms have been widely investigated, therapeutic options remain limited. One promising tool are mesenchymal stromal cells (MSCs). The aim this study to investigate the immunomodulatory effects human MSCs from two different sources (bone marrow/BMMSC and adipose tissue/ASC) evaluate cardioprotective potential. Methods 60 adult male C57BL/6 mice were divided into sham, sepsis (cecal ligation puncture (CLP)) i.v. treatment groups CLP + BMMSC ASC 5 animals in each group. observation periods 8, 24 72 h. Left ventricular tissue was analyzed histologically, by qPCR ( C3ar , C5ar1 Il-1b Il-6 Il-10 Tlr2 Tlr4 Tnfa Nlrp3 ) western blot. Cardiac damage markers troponin I heart fatty acid binding protein (HFABP) detected serum ELISA. Results Troponin HFABP significantly increased group after 8 h compared sham. In expression inflammasome upregulated up 24h After treatment, as well C5ar mRNA left ventricle downregulated CLP, whereas associated downregulation . Conclusions CLP-induced polymicrobial inflammation tissue. Therapeutic systemic application ameliorated heart.