Investigating the importance of B cells and antibodies during Trichuris muris infection using the IgMi mouse

• DOI: 10.1007/s00109-020-01954-3
• Publication Date: 2020-08-10T22:02:44Z
• Authors: Rinal Sahputra; Emma A Murphy; Ruth Forman; Iris Mair; Muhammad Z. H. Fa...; Ari Waisman; Werner Muller; Kathryn J. Else
• Keywords: Mesenteric lymph nodes; B-1 cell

Abstract The IgMi mouse has normal B cell development; its cells express an IgM receptor but cannot class switch or secrete antibody. Thus, the offers a model system by which to dissect out antibody-dependent and antibody-independent function. Here, we provide first detailed characterisation of post- Trichuris muris ( T. ) infection, describing expulsion phenotype, cytokine production, gut pathology changes in T regulatory cells, follicular helper germinal centre addition RNA sequencing (RNA seq) analyses wild-type littermates (WT) mutant prior post infection. mice were susceptible high-dose with reduced Th2 cytokines elevated cell-derived IL-10 mesenteric lymph nodes (MLN) compared controls. A low-dose infection regime revealed have significantly more apoptotic WT mice, no change intestinal inflammation. levels again elevated. Collectively, this study showcases potential as tool for understanding biology suggests that plays both roles high- Key messages During are important maintaining Th1/Th2 balance MLN through mechanism. High early post-infection, presence IL-10-producing correlates susceptibility maintain homeostasis during chronic via