QTL associations for density and diameter in Pinus radiata and the potential for marker-aided selection

• DOI: 10.1007/s00122-003-1446-2
• Publication Date: 2004-02-09T16:18:16Z
• Authors: M. E. Devey; S. D. Carson; M. F. Nolan; A. C. Matheson; C. Te Riini; J. Hohepa
• Keywords: Pinus radiata; Radiata; Marker-Assisted Selection; Association mapping; Tree breeding; Family-based QTL mapping

A large full-sib family of radiata pine ( Pinus radiata Donn. ex D. Don) was used for quantitative trait locus (QTL) detection and independent verification. QTL detection experiments were carried out for juvenile wood density (JWD) and stem diameter at breast height (DBH) using selective genotyping. Evenly spaced RFLP and microsatellite markers were selected from an existing linkage map. QTLs were verified in an independent set of progeny from the same family. Based on map location, at least eight QTL positions for JWD and two for DBH were detected and verified. The percent variance accounted for by the markers ranged from 0.78% to 3.58%, suggesting a genomic architecture of many genes with small effect. Two unrelated "bridging" families were chosen as candidates for marker-aided selection (MAS), and six microsatellite markers showing an association with JWD or DBH were tested in these families. Of these, four markers showed a consistent association with JWD in one or both of the bridging families. Results from this study provide a basis for MAS in P. radiata.