Mapping of extreme resistance to PVY (Ry sto) on chromosome XII using anther-culture-derived primary dihaploid potato lines

• DOI: 10.1007/s00122-005-0010-7
• Publication Date: 2005-07-25T10:37:53Z
• Authors: Ye-Su Song; Leonard Hepting; Günther Schweizer; Lorenz Hartl; Gerhard Wenzel; Andrea Schwarzfis...
• Keywords: 2. Zero hunger; 0301 basic medicine; 0303 health sciences; Base Sequence; DNA, Plant; Potyvirus; Chromosome Mapping; Flowers; Haploidy; Diploidy; Chromosomes, Plant; Immunity, Innate; 03 medical and health sciences; Disease Susceptibility; Plant Diseases; Solanum tuberosum

The inheritance of extreme resistance to PVY (Ry (sto)) by a single dominant locus was confirmed by obtaining a 1:1 segregation ratio in a virus inoculation test with 28 resistant (Ryry) to 29 susceptible (ryry) anther culture-derived dihaploid lines (2n=2x=24) from cv. "Assia" (2n=4x=48) having extreme resistance derived from Solanum stoloniferum in simplex constitution (Ryryryry). Twelve Ry (sto) markers selected in AFLP assays using bulked segregant analysis were applied to 106 tested potato cultivars from Germany, The Netherlands and Poland and 19 potato cultivars were identified by these markers as extremely resistant to PVY in alignment with phenotypic data. The locus for extreme resistance (Ry (sto)) to PVY was mapped on chromosome XII co-segregating with the SSR marker STM 0003. The utility of anther-culture derived dihaploid potatoes for genetic marker development was demonstrated. Marker transferability from diploids to tetraploids provides an optimistic potential for marker-assisted selection in potato breeding programs.