Comparative sequence analysis of the potato cyst nematode resistance locus H1 reveals a major lack of co-linearity between three haplotypes in potato (Solanum tuberosum ssp.)

• DOI: 10.1007/s00122-010-1472-9
• Publication Date: 2010-11-03T06:56:14Z
• Authors: Anna Finkers-Tomczak; Erin Bakker; Jan de Boer; Edwin van der Vossen; Ute Achenbach; Tomasz Golas; Suwardi Suryaningrat; Geert Smant; Jaap Bakker; Aska Goverse
• Keywords: Solanum tuberosum; Potato cyst nematode; Introgression

The H1 locus confers resistance to the potato cyst nematode Globodera rostochiensis pathotypes 1 and 4. It is positioned at distal end of chromosome V diploid Solanum tuberosum genotype SH83-92-488 (SH) on an introgression segment derived from S. ssp. andigena. Markers a high-resolution genetic map (Bakker et al. in Theor Appl Genet 109:146–152, 2004) were used screen BAC library construct physical covering 341-kb region resistant haplotype coming SH. For comparison, maps also generated two haplotypes susceptible RH89-039-16 (S. tuberosum/S. phureja), spanning syntenic regions 700 319 kb. Gene predictions genomic segments resulted identification large cluster consisting variable numbers CC-NB-LRR type R genes for each haplotype. Furthermore, interspersed with numerous transposable elements coding extensin-like protein amino acid transporter. Comparative analysis revealed major lack gene order conservation sequences three closely related haplotypes. Our data provide insight evolutionary mechanisms shaping will facilitate map-based cloning gene.