Fine mapping of qSTV11 KAS , a major QTL for rice stripe disease resistance

• DOI: 10.1007/s00122-011-1557-0
• Publication Date: 2011-03-08T06:11:47Z
• Authors: Ying-Xin Zhang; Qi Wang; Ling Jiang; Ling-Long Liu; Bao-Xiang Wang; Ying-Yue Shen; Xia-Nian Cheng; Jian-min Wan
• Keywords: Inbred strain

Rice stripe disease, caused by rice virus (RSV), is one of the most serious diseases in temperate rice-growing areas. In present study, we performed quantitative trait locus (QTL) analysis for RSV resistance using 98 backcross inbred lines derived from cross between highly resistant variety, Kasalath, and susceptible Nipponbare. Under artificial inoculation greenhouse, two QTLs resistance, designated qSTV7 qSTV11 KAS , were detected on chromosomes 7 11 respectively, whereas only QTL was same location chromosome under natural field. The stability validated 39 established segment substitution lines. Fine mapping carried out 372 BC3F2:3 recombinants 399 BC3F3:4 selected 7,018 BC3F2 plants SL-234/Koshihikari. localized to a 39.2 kb region containing seven annotated genes. likely candidate gene, LOC_Os11g30910, predicted encode sulfotransferase domain-containing protein. protein encoded Kasalath allele differs Nipponbare single amino acid deletion acids within domain. Marker-resistance association revealed that markers L104-155 bp R48-194 correlated with 148 landrace varieties. These results provide basis cloning may be used molecular breeding