Identification and fine mapping of a major locus controlling branching in Brassica napus
2. Zero hunger
0301 basic medicine
0303 health sciences
Genetic Linkage
Brassica napus
Quantitative Trait Loci
Chromosome Mapping
Genes, Recessive
Genomics
Genes, Plant
Polymorphism, Single Nucleotide
Chromosomes, Plant
03 medical and health sciences
Phenotype
Mutation
Crosses, Genetic
Genetic Association Studies
DOI:
10.1007/s00122-019-03506-x
Publication Date:
2019-12-16T10:20:31Z
AUTHORS (11)
ABSTRACT
A candidate branching-controlling gene for qDBA09 was identified after delimiting a Brassica napus recessive locus within a 270-kb interval on chromosome A09. Although branching is an important trait associated with the adaptation and yield potential of rapeseed (Brassica napus), the genetic mechanisms underlining branching in this crop remain poorly understood. In this study, we characterized a naturally occurring rapeseed mutant, db1, which showed an ultrahigh branching density phenotype. By combining bulked segregant analysis (BSA) and the Brassica 60K SNP BeadChip Array, we identified two major quantitative trait loci (QTLs), qDBA09 and qDBC06, which were subsequently confirmed using the traditional QTL-mapping approach. Analysis of 208 individuals from a BC1F3 population indicated that the qDBA09 locus is a single Mendelian factor and that the dense branching phenotype is controlled by a single recessive gene. Furthermore, QTL analysis confirmed that qDBA09 explained between 9.5 and 70.5% of the variation in branching-related traits. Using 7785 individuals from the BC1F3 population, we mapped qDBA09 to a DNA fragment of approximately 270 kb in length that contained 27 predicted genes, three of which were identified as potentially involved in the control of the dense branching trait. Based on the reported function of these genes, together with sequence comparisons and co-segregation analysis, we identified a potential candidate gene for the qDBA09 locus. The present findings lay the foundations for further in-depth research on the branching mechanisms of B. napus.
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CITATIONS (23)
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