Genotyping-by-sequencing uncovers a Thinopyrum 4StS·1JvsS Robertsonian translocation linked to multiple stress tolerances in bread wheat

• DOI: 10.1007/s00122-024-04791-x
• Publication Date: 2024-12-26T04:54:20Z
• Authors: Klaudia Kruppa; Edina Türkösi; Kateřina Holušová; Balázs Kalapos; Éva Szakács; Mónika Cséplő; András Farkas; László Ivanizs; Kitti Szőke-Pázsi; Péter Mikó; Péter Kovács; Andrea Gulyás; Norbert Hidvégi; Márta Molnár-Láng; Éva Darkó; Jan Bartoš; Eszter Gaál; István Molnár
• Keywords: Drought Tolerance; Robertsonian translocation; Common wheat; Stem rust

GBS read coverage analysis identified a Robertsonian chromosome from two Thinopyrum subgenomes in wheat, conferring leaf and stripe rust resistance, drought tolerance, maintaining yield stability. Agropyron glael (GLAEL), intermedium × Th. ponticum hybrid, serves as valuable genetic resource for wheat improvement. Despite its potential, limited knowledge of structure homoeologous relationships with hexaploid (Triticum aestivum) has restricted the full exploitation GLAEL's diversity breeding programs. Here, we present development 44-chromosome wheat/GLAEL addition line (GLA7). Multicolor genomic situ hybridization one arm St subgenome intermedium, while other remained unclassified. Genotyping-by-sequencing (GBS) revealed unique translocation between distinct subgenomes, 4StS·1JvsS. The GLA7 demonstrated strong adult plant resistance to both under natural artificial infection conditions. Automated phenotyping shoot morphological parameters together relative water content components showed that exhibited elevated tolerance compared parental genotypes. Three years field trials exhibits similar agronomic performance parents. This holds promise enhancing wheat's multiple stresses through introduction new genes, well ability mitigate effects temporary limitation during flowering, all without negatively impacting performance.