Haemocyte Apoptosis of the Tiger Shrimp Penaeus monodon Exposed to Cadmium

• DOI: 10.1007/s00128-013-1165-6
• Publication Date: 2013-12-27T10:33:09Z
• Authors: Jian-An Xian; Bin Li; Hui Guo; Yu-Tao Miao; Jian-Min Ye; Li-Na Feng; Chao-Xia Ye; Xun-Bin Pan; An-Li Wang; Xuan-Ming Hao
• Keywords: 0301 basic medicine; Oxidative Stress; 03 medical and health sciences; Hemocytes; Penaeidae; Animals; Apoptosis; 14. Life underwater; Reactive Oxygen Species; Water Pollutants, Chemical; Cadmium

This study investigated the effect of ambient Cadmium (Cd) on haemocyte apoptosis of the shrimp, Penaeus monodon. Cellular response was determined in Cd-exposed (0, 0.05, 0.5 and 5 mg L(-1)) shrimp. Results showed that 0.05 mg L(-1) Cd(2+) had no significant effect on the haemocyte parameters during the 48 h exposure. Cadmium at doses of 0.5 and 5 mg L(-1) depressed the total haemocyte count (THC), and increased reactive oxygen species (ROS) production and apoptosis ratio in haemocytes. Esterase activity increased in shrimp exposed to 0.5 mg L(-1) Cd(2+) for 6 h, and decreased to the initial level later. Depressed esterase activity could be observed in shrimp after 24 and 48 h exposure to 5 mg L(-1) Cd(2+). These results demonstrated that Cd(2+) modified esterase activity and induced ROS generation, which led to haemocyte apoptosis and THC reduction. Oxidative stress is one of the induction mechanisms for Cd-caused apoptosis of shrimp haemocytes.