The anti-diabetic drug metformin does not affect bone mass in vivo or fracture healing

• DOI: 10.1007/s00198-013-2371-0
• Publication Date: 2013-05-03T10:38:02Z
• Authors: J. Jeyabalan; B. Viollet; P. Smitham; S. A. Ellis; G. Zaman; C. Bardin; A. Goodship; J. P. Roux; M. Pierre; C. Chenu
• Keywords: [SDV]Life Sciences [q-bio]; Endocrinology, Diabetes and Metabolism; Ovariectomy; Bone architecture; micro-CT; AMP-Activated Protein Kinases; histomorphometry; Mice; 03 medical and health sciences; Bone Density; 616; Animals; Hypoglycemic Agents; Femur; Bony Callus; Rats, Wistar; Fracture Healing; 0303 health sciences; Tibia; X-Ray Microtomography; fracture healing; Metformin; Rats; 3. Good health; [SDV] Life Sciences [q-bio]; Enzyme Activation; Mice, Inbred C57BL; Osteoporosis; Original Article; Female; Bone Remodeling; metformin; Femoral Fractures

The present study shows no adverse effects of the anti-diabetic drug metformin on bone mass and fracture healing in rodents but demonstrates that is not osteogenic vivo, as previously proposed. In view increased incidence fractures patients with type 2 diabetes mellitus (T2DM), we investigated metformin, a widely used T2DM therapy, vivo using two different rodent models modes administration. We first subjected 12-week-old female C57BL/6 mice to ovariectomy (OVX). Four weeks after OVX, received either saline or administered by gavage (100 mg/kg/daily). After 4 treatment, micro-architecture cellular activity were determined tibia micro-CT histomorphometry. another experiment, Wistar rats aged 3 months given only water for 8 via drinking (2 mg/ml). mid-diaphyseal osteotomy was performed left femur. Rats sacrificed architecture analysed right while callus volume femur X-ray analysis micro-CT, respectively. both models, our results show significant differences cortical trabecular metformin-treated compared saline. Metformin had effect resorption reduced formation rate bone. Mean scores assessed control showed between groups. Fracture mineral content similar Our indicate has rodents.