Comparative transcriptome analysis of Trueperella pyogenes reveals a novel antimicrobial strategy

• DOI: 10.1007/s00203-017-1338-5
• Publication Date: 2017-01-31T13:34:02Z
• Authors: Kelei Zhao; Wujiao Li; Ting Huang; Xuhao Song; Xiuyue Zhang; Bisong Yue
• Keywords: 0301 basic medicine; Histidine Kinase; Virulence; Virulence Factors; Gene Expression Profiling; Quorum Sensing; 3. Good health; DNA-Binding Proteins; 03 medical and health sciences; Anti-Infective Agents; Biofilms; Actinomycetaceae; Pseudomonas aeruginosa; Escherichia coli; Animals; Female; Actinomycetales Infections

Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. Although the genomic information of this species has been announced, little is known about its functional profiles. In this study, by performing a comparative transcriptome analysis between the highly and moderately virulent T. pyogenes isolates, we found the expression of a LuxR-type DNA-binding response regulator, PloR, was significantly up-regulated in the highly virulent T. pyogenes. Protein crystal structure prediction and primary functional assessment suggested that, the quorum-sensing signal molecules of Gram-negative bacteria such as Pseudomonas aeruginosa and Escherichia coli could significantly inhibit the growth, biofilm production and hemolysis of T. pyogenes by binding to the upstream sensor histidine kinase, PloS. Therefore, the PloS/PlosR two-component regulatory system might dominate the virulence of T. pyogenes. Our findings provide a major advance in understanding the pathogenesis of T. pyogenes, and may shed new light on the development of novel therapeutic strategies to control T. pyogenes infection.