Identification of an antiviral compound isolated from Pistacia lentiscus

• DOI: 10.1007/s00203-020-01980-2
• Publication Date: 2020-07-15T17:04:00Z
• Authors: Lamjed Bouslama; Roudaina Benzekri; Siwar Nsaibia; Adele Papetti; Ferid Limam
• Keywords: Plant Extracts; Herpesvirus 2, Human; 610; Herpes simplex virus type 2; Dammaradienone; Antiviral Agents; Virucidal selectivity index; Adenoviridae; 3. Good health; Plant Leaves; 03 medical and health sciences; 0302 clinical medicine; Pistacia lentiscu; Pistacia; Seeds; Viruses; Solvents; dammaradienol; Chromatography, High Pressure Liquid; Enterovirus

This study screened mastic gum (Pistacia lentiscus L.) for antiviral activity against herpes simplex virus type 2 (HSV-2), coxsackievirus type B3, and adenovirus type 5. The organs of this plant (leaves, stem, and seed) were macerated sequentially using solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and methanol). Only the methanol extract of stem exhibited significant activity against HSV-2. This extract showed anti-HSV-2 activity with a selectivity index of 51 (50% cytotoxic concentration = 186 µg/mL; 50% inhibitory concentration = 3.63 µg/mL), and demonstrated direct inhibition against this virus with a virucidal selectivity index of 620 (50% virucidal concentration = 0.30 µg/mL). A bio-guided assay involving thin-layer chromatography led to the isolation of two active compounds, which have been identified as dammaradienone and dammaradienol using high-performance liquid chromatography-diode array detection coupled with electrospray ionization mass spectrometry. P. lentiscus has been widely studied for other biological activities. However, to our knowledge, this is the first report of P. lentiscus L. exhibiting antiviral activity.