The effects of triclosan on pluripotency factors and development of mouse embryonic stem cells and zebrafish
Pluripotency
0301 basic medicine
Embryo, Nonmammalian
Cell Survival
Medical Biotechnology
Induced Pluripotent Stem Cells
Cell Culture Techniques
Stem Cell Research - Embryonic - Non-Human
Embryonic Development
Apoptosis
Zebrafish embryos
Endocrine Disruptors
Regenerative Medicine
Toxicology
Developmental retardation
Mice
03 medical and health sciences
Genetics
Animals
SOX Transcription Factors
Zebrafish
Homeodomain Proteins
Nonmammalian
Biomedical and Clinical Sciences
SOXB1 Transcription Factors
Cell Cycle
MicroRNA
Mouse Embryonic Stem Cells
Pharmacology and Pharmaceutical Sciences
Nanog Homeobox Protein
Zebrafish Proteins
Stem Cell Research
Triclosan
Up-Regulation
Pharmacology and pharmaceutical sciences
Embryo
Biochemistry and cell biology
Mouse embryonic stem cells
Octamer Transcription Factor-3
DOI:
10.1007/s00204-014-1270-2
Publication Date:
2014-05-30T06:07:56Z
AUTHORS (9)
ABSTRACT
Triclosan (TCS) poses potential risks to reproduction and development due to its endocrine-disrupting properties. However, the mechanism of TCS's effects on early embryonic development is little known. Embryonic stem cells (ESC) and zebrafish embryos provide valuable models for testing the toxic effects of environmental chemicals on early embryogenesis. In this study, mouse embryonic stem cells (mESC) were acutely exposed to TCS for 24 h, and general cytotoxicity and the effect of TCS on pluripotency were then evaluated. In addition, zebrafish embryos were exposed to TCS from 2- to 24-h post-fertilization (hpf), and their morphology was evaluated. In mESC, alkaline phosphatase staining was significantly decreased after treatment with the highest concentration of TCS (50 μM). Although the expression levels of Sox2 mRNA were not changed, the mRNA levels of Oct4 and Nanog in TCS-treated groups were significantly decreased compared to controls. In addition, the protein levels of Oct4, Sox2 and Nanog were significantly reduced in response to TCS treatment. MicroRNA (miR)-134, an expression inhibitor of pluripotency markers, was significantly increased in TCS-treated mESC. In zebrafish experiments, after 24 hpf of treatment, the controls had developed to the late stage of somitogenesis, while embryos exposed to 300 μg/L of TCS were still at the early stage of somitogenesis, and three genes (Oct4, Sox2 and Nanog) were upregulated in treated groups when compared with the controls. The two models demonstrated that TCS may affect early embryonic development by disturbing the expression of the pluripotency markers (Oct4, Sox2 and Nanog).
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CITATIONS (31)
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