Application of a multi-layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e-liquid and its corresponding aerosol using an e-cigarette device with MESH™ technology

• DOI: 10.1007/s00204-019-02565-9
• Publication Date: 2019-09-07T07:02:45Z
• Authors: Anita R. Iskandar; Filippo Zanetti; Diego Marescotti; Bjorn Titz; Alain Sewer; Athanasios Kondylis; Patrice Leroy; Vincenzo Belcastro; Laura Ortega Torres; Stefano Acali; Shoaib Majeed; Sandro Steiner; Keyur Trivedi; Emmanuel Guedj; Celine Merg; Thomas Schneider; Stefan Frentzel; Florian Martin; Nikolai V. Ivanov; Manuel C. Peitsch; Julia Hoeng
• Keywords: Electronic cigarette

We previously proposed a systems toxicology framework for in vitro assessment of e-liquids. The starts with the first layer aimed at screening potential toxicity e-liquids, followed by second investigating toxicity-related mechanism and finally, third evaluating corresponding aerosols. In this work, we applied to assess impact e-liquid MESH Classic Tobacco its aerosol compared that cigarette smoke (CS) from 3R4F reference cigarette. layer, evaluated cytotoxicity profile (containing humectants, nicotine, flavors) Base humectant nicotine only) comparison total particulate matter (TPM) CS using primary bronchial epithelial cell cultures. same culture model was used explore changes specific markers high-content assays identify mechanisms induced e-liquids beyond viability TPM-induced effects. Finally, exposure or aerosols human organotypic air-liquid interface buccal small airway results showed liquid similar but lower than TPM comparable concentrations. Relative exposure, did not cause tissue damage elicited mRNA, microRNA, protein markers. context tobacco harm reduction strategy, is suitable potential-reduced electronic relative CS.