Formaldehyde-induced hematopoietic stem and progenitor cell toxicity in mouse lung and nose

• DOI: 10.1007/s00204-020-02932-x
• Publication Date: 2020-10-21T09:03:01Z
• Authors: Yun Zhao; Laura C. Magaña; Haiyan Cui; Jiawei Huang; Cliona M. McHale; Xu Yang; Mark R. Looney; Rui Li; Luoping Zhang
• Keywords: Male; 0301 basic medicine; Cells; Medical Biotechnology; 610; Bone Marrow Cells; HSC; Nose; Regenerative Medicine; Toxicology; Mice; 03 medical and health sciences; Rare Diseases; Stem Cell Research - Nonembryonic - Human; Cardiovascular Medicine and Haematology; Formaldehyde; Animals; Colony; Lung; Inbred BALB C; Cells, Cultured; HSC/HPC; Inhalation Exposure; Mice, Inbred BALB C; Cultured; Leukemogenesis; Leukemia; Biomedical and Clinical Sciences; Toxicity; Hematology; Pharmacology and Pharmaceutical Sciences; Stem Cell Research; Hematopoietic Stem Cells; Specific Pathogen-Free Organisms; 3. Good health; Pharmacology and pharmaceutical sciences; Biochemistry and cell biology; HPC; Carcinogens; Stem Cell Research - Nonembryonic - Non-Human; Spleen

Formaldehyde (FA), an economically important and ubiquitous chemical, has been classified as a human carcinogen and myeloid leukemogen. However, the underlying mechanisms of leukemogenesis remain unclear. Unlike many classical leukemogens that damage hematopoietic stem/progenitor cells (HSC/HPC) directly in the bone marrow, FA-as the smallest, most reactive aldehyde-is thought to be incapable of reaching the bone marrow through inhalation exposure. A recent breakthrough study discovered that mouse lung contains functional HSC/HPC that can produce blood cells and travel bi-directionally between the lung and bone marrow, while another early study reported the presence of HSC/HPC in rat nose. Based on these findings, we hypothesized that FA inhalation could induce toxicity in HSC/HPC present in mouse lung and/or nose rather than in the bone marrow. To test this hypothesis, we adapted a commercially available protocol for culturing burst-forming unit-erythroid (BFU-E) and colony-forming unit-granulocyte, macrophage (CFU-GM) colonies from bone marrow and spleen to also enable culture of these colonies from mouse lung and nose, a novel application of this assay. We reported that in vivo exposure to FA at 3 mg/m3 or ex vivo exposure up to 400 µM FA decreased the formation of both colony types from mouse lung and nose as well as from bone marrow and spleen. These findings, to the best of our knowledge, are the first empirically to show that FA exposure can damage mouse pulmonary and olfactory HSC/HPC and provide potential biological plausibility for the induction of leukemia at the sites of entry rather than the bone marrow.