Development and validation of a rapid LC–MS/MS method for the confirmatory analysis of the bound residues of eight nitrofuran drugs in meat using microwave reaction

• DOI: 10.1007/s00216-021-03763-0
• Publication Date: 2021-11-23T23:02:21Z
• Authors: Gemma Regan; Mary Moloney; Melissa Di Rocco; Padraig McLoughlin; Wesley Smyth; Steven Crooks; Christopher Elliott; Martin Danaher
• Keywords: Nitrofuran; Furazolidone; Nitrofurazone; QuEChERS; Sample Preparation; Solid phase extraction

Abstract A rapid analytical method was developed and validated for the analysis of eight bound nitrofurans in animal tissue, shortening laboratory turnaround times from 4 to 2 days. The majority methodologies nitrofuran focus on detection only four drugs (nitrofurantoin, furazolidone, furaltadone nitrofurazone), is time-consuming given 16-h overnight derivatisation step a double liquid–liquid extraction. In this study, narrow scope addressed by including further important (nifursol, nitrofuroxazide, nifuraldezone nitrovin). Full chromatographic separation achieved metabolites all nitrofurans, using phenyl-hexyl column chemistry rigorous optimisation mobile phase additives gradient profile. conventional, lengthy sample preparation substantially shortened replacing traditional water bath with 2-h microwave-assisted reaction, followed modified-QuEChERS (Quick, Easy, Cheap, Effective, Rugged Safe) This confirmatory fully accordance new 2021/808/EC legislation, shown perform satisfactorily when applied incurred tissues. decision limit (CCα) analytes ranged between 0.013 0.200 µg kg −1 , showing abundant sensitivity that current RPA 0.5 . innovative can play major role surveillance illegal use drugs.