An ultrasensitive hairpin sensor based on g-C3N4 nanocomposite for the detection of miRNA-155 in breast cancer patient serum

• DOI: 10.1007/s00216-022-04284-0
• Publication Date: 2022-08-16T22:02:31Z
• Authors: Pingping Peng; Sheng Wang; Siyuan Cai; Jing Cheng; Dan Tao; Nicole Jaffrezic-...; Zhenzhong Guo
• Keywords: 0301 basic medicine; Glucosamine; [CHIM.ANAL] Chemical Sciences/Analytical chemistry; Metal Nanoparticles; Breast Neoplasms; Biosensing Techniques; Electrochemical Techniques; Nanocomposites; MicroRNAs; 03 medical and health sciences; Limit of Detection; Reducing Agents; Nitriles; [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology; Biomarkers, Tumor; Humans; Female; Graphite; Gold; Nitrogen Compounds

Achieving the early diagnosis of breast cancer, through ultrasensitive detection of tumor marker miRNA-155, is a significant challenge. Therefore, an ultrasensitive hairpin electrochemical biosensor based on graphite-like phase carbon nitride composite was proposed. In this paper, poly(D-glucosamine) (PDG) was used as a stabilizer and reducing agent to prepare gold nanoparticles at room temperature, and then a graphite-like phase with a two-dimensional lamellar structure carbon nitride was further combined with it to obtain the poly(D-glucosamine)/gold nanoparticles/graphite-like phase carbon nitride nanocomposite (PDG/AuNPs/g-C3N4), in order to achieve the goal of signal amplification. The specific hairpin capture probe (HP) that recognized and bound miRNA-155 was then grafted. The hairpin biosensor showed a linear range of 0.1 fM-1 pM with a detection limit of 0.05 fM using differential pulse voltammetry (DPV) electrochemical analysis. Furthermore, the excellent performance hairpin electrochemical biosensor had been applied to the detection of miRNA-155 in human serum samples with good recovery.