Closing the gap — development of an analytical methodology using volumetric absorptive microsampling of finger prick blood followed by LC-HRMS/MS for adherence monitoring of antihypertensive drugs

• DOI: 10.1007/s00216-022-04394-9
• Publication Date: 2022-11-01T10:02:30Z
• Authors: Cathy M. Jacobs; Michael Kunz; Felix Mahfoud; Lea Wagmann; Markus R. Meyer
• Keywords: Lisinopril; Bioanalysis; Therapeutic Drug Monitoring; Amlodipine

Volumetric absorptive microsampling (VAMS), an emerging technique, is a promising tool for adherence monitoring. This study focused on development of analytical methodology to improve VAMS-based strategies assessment by analyzing angiotensin-converting-enzyme (ACE) inhibitors, loop diuretics, potassium-sparing diuretic, and thiazide diuretic. Development included sample preparation, chromatographic conditions, mass spectrometry settings, validation, demonstrating proof concept. Quantification analytes, name furosemide, hydrochlorothiazide, lisinopril, torasemide, the active metabolites, canrenone, enalaprilat, ramiprilat in finger prick blood (FPB), was validated based international guidelines. Selectivity, carryover, within/between-run accuracy precision were accordance with recommendations. The matrix effect evaluated at three different hematocrit levels (HT: 20%, 40%, 60%) coefficients variation did not exceed 15%. Dilution integrity (1:10 1:20) given all analytes except yet therapeutic range already covered calibration range. Long-term stability VAMS tips tested 2 weeks 24 °C dark revealed no degradation analytes. concept performed 35 intakes ACE-inhibitors diuretics 18 matched plasma samples. Hereby, determined concentration FPB cannot be used interchangeably, thus specific reference ranges whole must established. Nevertheless, strategy shown suitable assessing classes antihypertensive drugs guidelines manage hypertension.