Tunable lipid-coated nanoporous silver sheet for characterization of protein-membrane interactions by surface-enhanced Raman scattering (SERS)

• DOI: 10.1007/s00216-023-04701-y
• Publication Date: 2023-04-21T13:03:22Z
• Authors: Hongni Zhu; Jianing Zhang; Xin Dai; Vince St. Dollent...; Huanyu Chi; Congcheng Wang; Chi Shun Yeung; Qing Chen; Wei Liu; Jinqing Huang
• Keywords: Nanoporous; Model lipid bilayer; Peripheral membrane protein; Hydrophobic effect

Abstract Membrane environments affect protein structures and functions through protein-membrane interactions in a wide range of important biological processes. To better study the effects from lipid’s hydrophilic hydrophobic interaction with on different membrane regions, we developed lipid-coated nanoporous silver sheets to provide tunable supported lipid monolayer/bilayer for situ surface-enhanced Raman vibrational spectroscopy (SERS) characterizations. Under controllable surface pressure, was coated along microscopic curved serve as cell mimic well barrier avoid denaturation while empowering high SERS enhancements underlying metallic bases allowing detection sensitivity at low physiological concentrations. Moreover, fine-tuned packing density controlled orientation deposited bilayers monolayers directly monitor upon various parts/positions. Our results indicate that lysozyme adopted α-helical structure both membrane. Interestingly, alpha-synuclein folded into negatively charged heads, whereas tails induced β-sheet structural conversion originated its unstructured monomers. These direct observations might profound insights formation β-sheet-containing oligomers further disruptions amyloid genesis associated Parkinson’s disease. Hence, controllability tunability environments, our platform holds great promise more general applications investigating influences membranes correlative proteins under effects. Graphical