Cost-effective, user-friendly detection and preconcentration of thrombin on a sustainable paper-based electrochemical platform

• DOI: 10.1007/s00216-025-05764-9
• Publication Date: 2025-02-01T05:11:10Z
• Authors: Ada Raucci; Giuseppina Sorren...; Sima Singh; Nicola Borbone; Giorgia Oliviero; Gennaro Piccialli; Monica Terracciano; Stefano Cinti
• Keywords: User Friendly

Thrombin overexpression in serum serves as a critical biomarker and is implicated several diseases associated with significant morbidity mortality. Existing techniques for thrombin detection are time-consuming require sophisticated equipment extensive sample preparation procedures, which further delay the increase cost of procedure. Early accessible diagnosis at point care, especially limited-resource countries, represents first step clinical interventions. To overcome these limitations, we have proposed an innovative, sustainable paper-based electrochemical platform thrombin. In this work, aptasensor was rationally designed, characterized, evaluated against conventional gold standard plastic-based substrates, applied to human serum, yielding limit ~ 60 pM. The present method provides efficient user-friendly way potentially leading better management treatment outcomes patients.