Production of dihydroxyacetone from an aqueous solution of glycerol in the reaction catalyzed by an immobilized cell preparation of acetic acid bacteria Gluconobacter oxydans ATCC 621

• DOI: 10.1007/s00217-012-1846-0
• Publication Date: 2012-12-22T16:33:24Z
• Authors: Stasiak-Różańska ...; Błażejak Stanisław
• Keywords: Dihydroxyacetone; Acetic acid bacteria; Biocatalysis

Dihydroxyacetone (1,3-dihydroxy-2-propanone, DHA) is applied in the food and cosmetic industries as well pharmacy medicine. It produced a result of incomplete oxidation glycerol by acetic acid bacteria Gluconobacter oxydans. This reaction catalyzed PQQ-dependent membrane-bound dehydrogenase. The research developed method obtaining DHA 3 % aqueous solution (pH 7.5) at temperature 23 °C, with only biocatalyst being an immobilized cell preparation obtained from G. oxydans cells. After 5 days process, concentration accounted for 27.2 g/L efficiency 94 %. 4 run culture media pH 5.0, 28 free or cells on average 25 g DHA/L 87