Selection on GGU and CGU Codons in the High Expression Genes in Bacteria

• DOI: 10.1007/s00239-013-9596-6
• Publication Date: 2013-11-22T07:48:00Z
• Authors: Siddhartha Sankar...; Bhesh Raj Powdel; Malay Dutta; Alak Kumar Burago...; Suvendra Kumar Ray
• Keywords: Base Composition; 0303 health sciences; 03 medical and health sciences; Amino Acid Substitution; Bacteria; Nucleotides; Nucleotide Mapping; Amino Acids; Selection, Genetic; Codon

The fourfold degenerate site (FDS) in coding sequences is important for studying the effect of any selection pressure on codon usage bias (CUB) because nucleotide substitution per se is not under any such pressure at the site due to the unaltered amino acid sequence in a protein. We estimated the frequency variation of nucleotides at the FDS across the eight family boxes (FBs) defined as Um(g), the unevenness measure of a gene g. The study was made in 545 species of bacteria. In many bacteria, the Um(g) correlated strongly with Nc'-a measure of the CUB. Analysis of the strongly correlated bacteria revealed that the U-ending codons (GGU, CGU) were preferred to the G-ending codons (GGG, CGG) in Gly and Arg FBs even in the genomes with G+C % higher than 65.0. Further evidence suggested that these codons can be used as a good indicator of selection pressure on CUB in genomes with higher G+C %.