Antibiotic Activity of Wickerhamomyces anomalus Mycocins on Multidrug-Resistant Acinetobacter baumannii

• DOI: 10.1007/s00248-020-01495-9
• Publication Date: 2020-02-18T18:02:50Z
• Authors: Daniele S. B. Junges; Mateus F. Delabeneta; Lana Rubia B. Ros...; Bruna L. Nascimento; Ana Paula Paris; Cristiane Persel; Eduardo A. Loth; Rita C. G. Simão; Rafael A. Menolli; Claudete R. Paula; Rinaldo F. Gandra
• Keywords: Acinetobacter baumannii; 0301 basic medicine; 03 medical and health sciences; Erythrocytes; Anti-Infective Agents; Saccharomycetales; Animals; Humans; Artemia; 3. Good health

To evaluate the susceptibility of multidrug-resistant Acinetobacter baumannii to mycocins produced by Wickerhamomyces anomalus and to verify the cytotoxicity of these compounds. Three culture supernatants of W. anomalus (WA40, WA45, and WA92), containing mycocins (WA40M1, WA45M2, and WA92M3), were tested on A. baumannii using broth microdilution methods, solid medium tests, and cytotoxicity tests in human erythrocytes and in Artemia saline Leach. W. anomalus was able to produce high antimicrobial mycocins, as even at high dilutions, they inhibited A. baumannii. In a solid medium, it was possible to observe the inhibition of A. baumannii, caused by the diffusion of mycocins between agar. Finally, the three supernatants were not cytotoxic when tested on human erythrocytes and Artemia salina. According to the evidence in this study, the mycocins of W. anomalus have been effective and could be used in the development of new antimicrobial substances.