Incidence and Diversity of Torix Rickettsia–Odonata Symbioses

• DOI: 10.1007/s00248-020-01568-9
• Publication Date: 2020-08-07T16:03:11Z
• Authors: Panupong Thongprem; Helen R. Davison; David J. Thompson; M. Olalla Lorenzo...; Gregory D. D. Hurst
• Keywords: Coenagrionidae; Damselfly; Introgression

Abstract Heritable microbes are an important component of invertebrate biology, acting both as beneficial symbionts and reproductive parasites. Whilst most previous research has focussed on the ‘ Wolbachia pandemic’, recent work emphasised importance other microbial symbionts. In this study, we present a survey odonates (dragonflies damselflies) for torix group Rickettsia , following indicating that clade can be common in aquatic insect groups. PCR assays were used to screen broad range from two continents revealed 8 76 species tested infected with . We then conducted further deeper screening UK representatives Coenagrionidae damselfly family, revealing 6 coenagrionid positive Analysis gene sequences supported multiple establishments symbiosis group. Some strains shared between mtDNA barcodes, likely route mitochondrial introgression sister species. There was also evidence coinfecting FISH analysis indicated observed ovarioles, consistent heritable symbiosis. conclude represent associate odonates, being found Europe South America. is coinfection occur, vertical transmission likely, symbiont movement hybridisation may underpin lack ‘barcoding gap’ well-established pairs genus. Future should establish biological significance symbioses observed.