Faecal Microbiota Divergence in Allopatric Populations of Podarcis lilfordi and P. pityusensis, Two Lizard Species Endemic to the Balearic Islands

• DOI: 10.1007/s00248-022-02019-3
• Publication Date: 2022-04-28T10:05:12Z
• Authors: Iris Alemany; Ana Pérez-Cembranos; Valentín Pérez-Me...; José A. Castro; Antonia Picornell; Cori Ramon; José A. Jurado-Ri...
• Keywords: 0303 health sciences; Microbiota; Lizards; 15. Life on land; Podarcis lilfordi; Podarcis pityusensis; Allopatric populations; Host-microbiome interactions; 24 Ciencias de la vida; Feces; 03 medical and health sciences; 2401.16 Herpetología; Balearic Islands; Spain; Host Microbe Interactions; RNA, Ribosomal, 16S; Faecal microbiota; Animals; 14. Life underwater

Abstract Gut microbial communities provide essential functions to their hosts and are known to influence both their ecology and evolution. However, our knowledge of these complex associations is still very limited in reptiles. Here we report the 16S rRNA gene faecal microbiota profiles of two lizard species endemic to the Balearic archipelago (Podarcis lilfordi and P. pityusensis), encompassing their allopatric range of distribution through a noninvasive sampling, as an alternative to previous studies that implied killing specimens of these IUCN endangered and near-threatened species, respectively. Both lizard species showed a faecal microbiome composition consistent with their omnivorous trophic ecology, with a high representation of cellulolytic bacteria taxa. We also identified species-specific core microbiota signatures and retrieved lizard species, islet ascription, and seasonality as the main factors in explaining bacterial community composition. The different Balearic Podarcis populations are characterised by harbouring a high proportion of unique bacterial taxa, thus reinforcing their view as unique and divergent evolutionary entities.