Biotransformation of isoeugenol to vanillin by Pseudomonas putida IE27 cells

• DOI: 10.1007/s00253-006-0569-1
• Publication Date: 2006-08-29T11:44:33Z
• Authors: Mamoru Yamada; Yukiyoshi Okada; Toyokazu Yoshida; Toru Nagasawa
• Keywords: Isoeugenol; Pseudomonas putida; Vanillic acid; Biotransformation; Arthrobacter; Pseudomonas fluorescens

The ability to produce vanillin and/or vanillic acid from isoeugenol was screened using resting cells of various bacteria. The vanillin- and/or vanillic-acid-producing activities were observed in strains belonging to the genera Achromobacter, Aeromonas, Agrobacerium, Alcaligenes, Arthrobacter, Bacillus, Micrococcus, Pseudomonas, Rhodobacter, and Rhodococcus. Strain IE27, a soil isolate showing the highest vanillin-producing activity, was identified as Pseudomonas putida. We optimized the culture and reaction conditions for vanillin production from isoeugenol using P. putida IE27 cells. The vanillin-producing activity was induced by adding isoeugenol to the culture medium but not vanillin or eugenol. Under the optimized reaction conditions, P. putida IE27 cells produced 16.1 g/l vanillin from 150 mM isoeugenol, with a molar conversion yield of 71% at 20 degrees C after a 24-h incubation in the presence of 10% (v/v) dimethyl sulfoxide.