Vertical distribution of nitrite-dependent anaerobic methane-oxidising bacteria in natural freshwater wetland soils
DNA, Bacterial
0301 basic medicine
China
0303 health sciences
Bacteria
Molecular Sequence Data
Fresh Water
Sequence Analysis, DNA
15. Life on land
Biota
Polymerase Chain Reaction
6. Clean water
03 medical and health sciences
Bacterial Proteins
Isotope Labeling
Wetlands
Cluster Analysis
Anaerobiosis
Methane
Nitrites
Phylogeny
Soil Microbiology
DOI:
10.1007/s00253-014-6031-x
Publication Date:
2014-09-22T01:21:31Z
AUTHORS (10)
ABSTRACT
Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered process that is catalysed by "Candidatus Methylomirabilis oxyfera". In the present study, the vertical distribution (0-10, 20-30, 50-60 and 90-100 cm) of M. oxyfera-like bacteria was investigated in Xiazhuhu wetland, the largest natural wetland on the southern Yangtze River (China). Phylogenetic analyses showed that group A of M. oxyfera-like bacteria and pmoA genes occurred primarily at depths of 50-60 and 90-100 cm. Quantitative PCR further confirmed the presence of M. oxyfera-like bacteria in soil cores from different depths, with the highest abundance of 5.1 × 10(7) copies g(-1) dry soil at depth of 50-60 cm. Stable isotope experiments demonstrated that the n-damo process occurred primarily at depths of 50-60 and 90-100 cm, with the potential rates ranging from 0.2 to 14.5 nmol CO2 g(-1) dry soil d(-1). It was estimated that the methane flux may increase by approximately 2.7-4.3% in the examined wetland in the absence of n-damo. This study shows that the deep wetland soils (50-60 and 90-100 cm) are the preferred habitats for M. oxyfera-like bacteria. The study also highlights the potential importance of these bacteria in the methane and nitrogen cycles in deep wetland soils.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (46)
CITATIONS (62)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....