Vertical distribution of nitrite-dependent anaerobic methane-oxidising bacteria in natural freshwater wetland soils

DNA, Bacterial 0301 basic medicine China 0303 health sciences Bacteria Molecular Sequence Data Fresh Water Sequence Analysis, DNA 15. Life on land Biota Polymerase Chain Reaction 6. Clean water 03 medical and health sciences Bacterial Proteins Isotope Labeling Wetlands Cluster Analysis Anaerobiosis Methane Nitrites Phylogeny Soil Microbiology
DOI: 10.1007/s00253-014-6031-x Publication Date: 2014-09-22T01:21:31Z
ABSTRACT
Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered process that is catalysed by "Candidatus Methylomirabilis oxyfera". In the present study, the vertical distribution (0-10, 20-30, 50-60 and 90-100 cm) of M. oxyfera-like bacteria was investigated in Xiazhuhu wetland, the largest natural wetland on the southern Yangtze River (China). Phylogenetic analyses showed that group A of M. oxyfera-like bacteria and pmoA genes occurred primarily at depths of 50-60 and 90-100 cm. Quantitative PCR further confirmed the presence of M. oxyfera-like bacteria in soil cores from different depths, with the highest abundance of 5.1 × 10(7) copies g(-1) dry soil at depth of 50-60 cm. Stable isotope experiments demonstrated that the n-damo process occurred primarily at depths of 50-60 and 90-100 cm, with the potential rates ranging from 0.2 to 14.5 nmol CO2 g(-1) dry soil d(-1). It was estimated that the methane flux may increase by approximately 2.7-4.3% in the examined wetland in the absence of n-damo. This study shows that the deep wetland soils (50-60 and 90-100 cm) are the preferred habitats for M. oxyfera-like bacteria. The study also highlights the potential importance of these bacteria in the methane and nitrogen cycles in deep wetland soils.
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