Antiviral activity and specific modes of action of bacterial prodigiosin against Bombyx mori nucleopolyhedrovirus in vitro

• DOI: 10.1007/s00253-015-7242-5
• Publication Date: 2015-12-21T04:33:26Z
• Authors: Wei Zhou; Cheng Zeng; RenHua Liu; Jie Chen; Ru Li; XinYan Wang; WenWen Bai; XiaoYuan Liu; TingTing Xiang; Lin Zhang; YongJi Wan
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Prodigiosin; Animals; Bombyx; Virus Replication; Antiviral Agents; Nucleopolyhedroviruses; Cell Line; 3. Good health

Prodigiosin, the tripyrrole red pigment, is a bacterial secondary metabolite with multiple bioactivities; however, the antiviral activity has not been reported yet. In the present study, we found the antiviral activity of bacterial prodigiosin on Bombyx mori nucleopolyhedrovirus (BmNPV)-infected cells in vitro, with specific modes of action. Prodigiosin at nontoxic concentrations selectively killed virus-infected cells, inhibited viral gene transcription, especially viral early gene ie-1, and prevented virus-mediated membrane fusion. Under prodigiosin treatment, both progeny virus production and viral DNA replication were significantly inhibited. Fluorescent assays showed that prodigiosin predominantly located in cytoplasm which suggested it might interact with cytoplasm factors to inhibit virus replication. In conclusion, the present study clearly indicates that prodigiosin possesses significant antiviral activity against BmNPV.