Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli

• DOI: 10.1007/s00253-017-8641-6
• Publication Date: 2017-11-20T20:45:30Z
• Authors: David J. Wurm; Julian Quehenberger; Julia Mildner; Britta Eggenreich; Christoph Slouka; Andreas Schwaighofer; Karin Wieland; Bernhard Lendl; Vignesh Rajamanickam; Christoph Herwig; Oliver Spadiut
• Keywords: Inclusion bodies; Downstream processing

Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive aggregates of misfolded protein, and thus should be avoided during recombinant protein production, numerous biopharmaceutically important proteins currently produced as IBs. To obtain correctly folded, soluble product, IBs have to processed, namely, harvested, solubilized, refolded. Several years ago, it was discovered that, depending on cultivation conditions properties, contain partially folded structures, which makes IB processing more efficient. Here, we present a method tailored induction production E. by mixed feed system using glucose lactose its impact formation. Our allows tuning amount, size, size distribution, purity, does not facilitate processing, but is also crucial for potential direct applications nanomaterials biomaterials regenerative medicine.