Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4

• DOI: 10.1007/s00253-022-12037-3
• Publication Date: 2022-06-24T01:02:21Z
• Authors: Adelaide Braga; Daniela Gomes; João Rainha; Beatriz B. Cardoso; Cláudia Amorim; Sara C. Silvério; María Fernández-L...; Joana L. Rodrigues; Lígia R. Rodrigues
• Keywords: 0301 basic medicine; Sucrose; Zymomonas; Science & Technology; Schwanniomyces occidentalis (Ffase-Leu196); Ethanol; Zymomonas mobilis; Oligosaccharides; Levansucrase (sacB); 6-kestose; 03 medical and health sciences; Fructooligosaccharides; Fermentation; One-step fermentation; Fructooligosaccharides (FOS)

Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB, that was able to produce 73.4 ± 1.6 g L−1 of FOS, with a productivity of 1.53 ± 0.03 g L-1 h-1 and a yield of 0.31 ± 0.03 gFOS gsucrose-1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process.