Effects of Leishmania major infection on the gut microbiome of resistant and susceptible mice

• DOI: 10.1007/s00253-024-13002-y
• Publication Date: 2024-01-19T08:02:23Z
• Authors: Jakub Mrázek; Lucie Mrázková; Chahrazed Mekadim; Taťána Jarošíková; Imtissal Krayem; Yahya Sohrabi; Peter Demant; Marie Lipoldová
• Keywords: Genomics, Transcriptomics, Proteomics; 0301 basic medicine; 03 medical and health sciences

Abstract Cutaneous leishmaniasis, a parasitic disease caused by Leishmania major , is widely frequent form in humans. To explore the importance of host gut microbiota and to investigate its changes during L. infection, two different groups mouse models were assessed. The microbiome parts gut—ileum colon—from infected non-infected mice characterised sequencing 16S rDNA using an Ion Torrent PGM platform. Microbiome analysis was performed reveal related susceptibility genetics strains compartments compare results between mice. showed that infection affects mainly ileum microbiota, whereas colon bacterial community more stable. Different biomarkers determined resistant susceptible LEfSe analysis. Lactobacillaceae associated with resistance all . Genes xenobiotic biodegradation metabolism amino acid primarily enriched small intestine strains, while genes carbohydrate glycan biosynthesis most abundant These should improve our understanding host-parasite interaction provide important insights into effect leishmaniasis on microbiota. Also, this study highlights role genetic variation shaping diversity composition microbiome. Key points • Leishmaniasis may affect Biomarkers or Several pathways predicted be upregulated Graphical