Effects of inoculation and dry matter content on microbiome dynamics and metabolome profiling of sorghum silage

• DOI: 10.1007/s00253-024-13096-4
• Publication Date: 2024-03-08T07:01:27Z
• Authors: Zohreh Akhavan Kh...; Dongmei Xu; Rina Su; Xusheng Guo
• Keywords: Silage; Metabolome

Abstract Sorghum forage was ensiled for 90 days at two dry matter (DM) contents (27 vs. 39%) without or with Lactiplantibacillus plantarum inoculation. On day of fermentation, silages were sampled to assess the microbial community dynamics and metabolome profile. L. inoculation improved silage quality, as shown by a lower pH greater acetic acid concentration. Loss DM remained unaffected but in low- high-DM sorghum (14.4 6.62%). The microbiome analysis revealed that Pseudomonas congelans represented dominant species epiphytic microbiota both before ensiling. However, buchneri end Ensiling fermentation resulted distinct metabolic changes varying content. In low-DM silages, ensiling led accumulation 24 metabolites reduction relative concentration 13 metabolites. an increase 26 decrease 8 Compared non-inoculated increased 3 reduced 5 silages. Similarly, there elevation metabolites, while 7 other Ten bio-functional activity identified, including chrysoeriol, isorhamnetin, petunidin 3-glucoside, apigenin, caffeic acid, gallic p -coumaric trans-cinnamic herniarin, 3,4-dihydroxy-trans-cinnamate. This study presents comprehensive profiling during function content inoculation, particular emphasis on identifying may possess properties. Key points • loss not different higher silage. dominated ensiling, regardless level. 10 identified.