Using multi-omics to explore the effect of Bacillus velezensis SAAS-63 on resisting nutrient stress in lettuce

• DOI: 10.1007/s00253-024-13153-y
• Publication Date: 2024-04-29T13:02:06Z
• Authors: Yinshuang Bai; Ke Song; Mengxiang Gao; Juan Ma; Yifan Zhou; Hua Liu; Haijuan Zeng; Jinbin Wang; Xianqing Zheng
• Keywords: Biofertilizer; Beneficial organism

Abstract To avoid the unreasonable use of chemical fertilizer, an environmentally friendly means improving soil fertility is required. This study explored role plant growth-promoting rhizosphere bacteria (PGPR) strain Bacillus velezensis SAAS-63 in nutrient stress lettuce. Compared with no inoculation, B. inoculants exhibited significantly increased fresh weight, root length, and shoot height under deficiency, as well improved antioxidant activities proline contents. The exogenous addition also accumulation macroelements micronutrients elucidate resistance mechanisms induced by stress, high-throughput sequencing multi-omics analysis were performed. Inoculation altered microbial community relative abundances Streptomyces , Actinoallomurus Verrucomicrobia Chloroflexi. It worth noting that inoculant can affect metabolism. changed metabolic flow phenylpropanoid pathway deficiency promoted phenylalanine to participate more synthesis lignin precursors coumarin substances inhibiting flavone isoflavone, thus resistance. showed could help plants recruit microorganisms decompose utilize trehalose re-established carbon metabolism rhizosphere. Additionally, microbes found be closely related metabolites based on correlation analysis. results indicated PGPRs has important regulating metabolism, providing valuable information for understanding how complex biological processes enhance adaptation deficiency. Key points • growth nutrient-deficient conditions affected diversity structure synthesize resist Graphical abstract