Enhancement of immune responses to classical swine fever virus E2 in mice by fusion or mixture with the porcine IL-28B

• DOI: 10.1007/s00253-024-13399-6
• Publication Date: 2025-02-13T08:54:57Z
• Authors: Hong Yuan; Yunjuan Jiao; Jie Gao; Tao Wang; Yingju Xia; Kun Li; Yuxuan Yang; Jing Zhang; Huifang Bao; Lihao Wang; Pu Sun; Dong Li; Pinghua Li; Yimei Cao; Zhixun Zhao; Zaixin Liu; Zengjun Lu; Yebing Liu; Xingwen Bai
• Keywords: Classical swine fever

The E2 subunit vaccine has been considered a promising alternative to an attenuated classical swine fever (CSF) vaccine. However, it fails induce good cellular immune response. Given that immunogenic adjuvants can regulate the immunity achieve maximum efficacy against antigens, immunostimulatory effects of porcine IL-28B on CSF virus (CSFV) were evaluated in present study. We expressed recombinant proteins E2-IL28B, E2, and using CHO-S mammalian cells as antigen expression platform, three types CSFV vaccines based antigens + IL-28B, prepared, respectively. found both E2-IL28B exhibited superior immunogenicity with dramatically induced antibody titers neutralizing levels than alone. Moreover, or instead boosted responses via obviously increasing percentages CD3+CD4+ T lymphocytes, promoting lymphocyte proliferations, enhancing release Th1-type cytokines. All results revealed inclusion whether fused mixed significantly elevated E2-induced potencies, suggesting could be used molecular adjuvant optimize design for more effective controls disease. KEY POINTS: • New candidates developed which was immunoadjuvant potency This study provided novel insights into immunoregulatory properties optimized design.