Ginsenoside Rb1 prevents interleukin-1 beta induced inflammation and apoptosis in human articular chondrocytes

• DOI: 10.1007/s00264-013-1990-6
• Publication Date: 2013-07-08T02:58:16Z
• Authors: Wendan Cheng; Dongying Wu; Qiang Zuo; Zhen Wang; Weimin Fan
• Keywords: Aggrecan; Type II collagen

Osteoarthritis (OA) is an age-related joint disease that characterised by the degeneration of articular chondrocytes. Ginsenosides, most important pharmacological ingredients ginseng, have been proven to provide effective therapy for neurodegenerative diseases and can inhibit cell apoptosis. We investigated whether ginsenoside Rb1 modulate inflammation apoptosis in human chondrocytes.Chondrocytes were isolated from OA patients undergoing total knee replacement surgery. Apoptosis was assessed TUNEL (terminal deoxyribonucleotide transferasemediated dUTP nick end-labelling)-positive staining. Levels PGE2 NO(2)- detected ELISA. Gene expression levels measured type II collagen (Col2A1), aggrecan, MMP-13, COX-2, iNOS, caspase-3, PARP.The results showed TUNEL-positive staining chondrocytes decreased compared with IL-1β. Both 10 or 100 μg/ml inhibited effect IL-1β on decreasing PGE2, NO(2)-, caspase-3 PARP increasing aggrecan Col2A1 gene levels, block IL-1β-induced apoptosis.The suggest possesses potential anti-inflammatory anti-apoptotic properties chondrocytes, possibly binding oestrogen receptors exert its effects.