Moderate temperature reduction is sufficient for prevention of 5-fluorouracil-induced oral mucositis: an experimental in vivo study in rats

• DOI: 10.1007/s00280-022-04495-3
• Publication Date: 2022-12-01T05:00:28Z
• Authors: J. Walladbegi; M. Dankis; Ö. Aydogdu; M. Jontell; M. Winder
• Keywords: Mucositis; Cryotherapy; Proinflammatory cytokine; Oral mucosa; Bolus (digestion)

Abstract Purpose The current idea of how oral mucositis (OM) develops is primarily based on hypotheses and the early events which precede clinically established OM remain to be demonstrated. Cryotherapy (CT) continues have considerable promise in clinical settings reduce chemotherapy-induced OM. Although being effective, knowledge scarce regarding ideal temperature for prevention Thus, present study had two main objectives: (i) develop an animal model investigate OM; (ii) at what cooling these could abolished. Methods Male Sprague–Dawley rats were anaesthetized given intravenous bolus dose with cytostatic drug fluorouracil (5-FU). During first hour following injection 5-FU, cavity was cooled a mucosal range 15–30 ○ C, or left uncooled (35 C), serving as control. After 3–5 days, euthanized, buccal mucosa excised. Subsequently, thickness expression IL-6 TNF-α analyzed immunohistochemistry enzyme-linked immunosorbent assay (ELISA). Results Five days treatment statistically significant thickening occurred, distinct both observed. cryo-treated groups (15–30 °C) displayed significantly thinner compared control group °C). ELISA showed increase proinflammatory cytokines tissues exposed 5-FU that treated increasing temperatures Conclusion Bolus i.v. can used create functional Further, moderate reduction sufficient may