cAMP Receptor Protein of Salmonella enterica Serovar Typhimurium Modulate Glycolysis in Macrophages to Induce Cell Apoptosis

• DOI: 10.1007/s00284-018-1574-1
• Publication Date: 2018-10-12T14:35:37Z
• Authors: Ke Ding; Chunjie Zhang; Jing Li; Songbiao Chen; Chengshui Liao; Xiangchao Cheng; Chuang Yu; Zuhua Yu; Yanyan Jia
• Keywords: Salmonella typhimurium; 0301 basic medicine; Caspase 8; 0303 health sciences; Cyclic AMP Receptor Protein; Caspase 3; Macrophages; Apoptosis; Caspase 9; Cell Line; Mice; 03 medical and health sciences; Adenosine Triphosphate; RAW 264.7 Cells; Hexokinase; Pyruvic Acid; Animals; Lactic Acid; Glycolysis

We studied the role of glycolysis in the mechanism of cAMP receptor protein-induced macrophage cell death of Salmonella enterica serovar Typhimurium (S. Typhimurium). Cell apoptosis, caspase-3, -8, -9 enzyme activity, and pyruvic acid, lactic acid, ATP, and hexokinase (HK) contents were determined after infection of macrophages with S. Typhimurium SL1344 wild-type and a cAMP receptor protein mutant strain. While cell apoptosis, caspase-3, -8, -9 enzyme activity, lactic acid, hexokinase, and ATP levels significantly changed by infection with crp mutants compared to the wild-type strain (P < 0.05). Our data suggest that the cAMP receptor protein of S. Typhimurium can modulate macrophage death by effecting glycolysis levels. This finding may help to elucidate the mechanisms of S. Typhimurium pathogenesis.