Cathepsin B degrades RbcL during freezing-induced programmed cell death in Arabidopsis
0301 basic medicine
0303 health sciences
Arabidopsis Proteins
Ribulose-Bisphosphate Carboxylase
Freezing
Arabidopsis
Apoptosis
Cathepsin B
DOI:
10.1007/s00299-023-03099-2
Publication Date:
2024-02-28T17:02:29Z
AUTHORS (8)
ABSTRACT
Abstract Programmed cell death (PCD) has been well documented in both development and in response to environmental stresses in plants; however, PCD induced by freezing stress and its molecular mechanisms remain poorly understood. In the present study, we characterised freezing-induced PCD and explored its mechanisms in Arabidopsis. PCD induced by freezing stress was similar to that induced by other stresses and senescence in Arabidopsis plants with cold acclimation. Inhibitor treatment assays and immunoblotting indicated that cathepsin B mainly contributed to increased caspase-3-like activity during freezing-induced PCD. Cathepsin B was involved in freezing-induced PCD and degraded the large subunit, RbcL, of Rubisco. Our results demonstrate an essential regulatory mechanism of cathepsin B for Rubisco degradation in freezing-induced PCD, improving our understanding of freezing-induced cell death and nitrogen and carbohydrate remobilisation in plants.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (48)
CITATIONS (0)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....