Evaluation of the inflammatory markers CCL8, CXCL5, and LIF in patients with anastomotic leakage after colorectal cancer surgery

• DOI: 10.1007/s00384-020-03582-2
• Publication Date: 2020-04-19T11:02:37Z
• Authors: F. Klupp; S. Schuler; C. Kahlert; N. Halama; C. Franz; P. Mayer; T. Schmidt; A. Ulrich
• Keywords: Chemokine CXCL5; Anastomosis, Surgical; Anastomotic Leak; Leukemia Inhibitory Factor; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Chemokine CCL8; Humans; Original Article; Prospective Studies; Colorectal Neoplasms; CXCL5 ; LIF ; CCL8 ; Humans [MeSH] ; Prospective Studies [MeSH] ; Colorectal Neoplasms/surgery [MeSH] ; Leukemia Inhibitory Factor [MeSH] ; Colorectal cancer ; Chemokine CXCL5 [MeSH] ; Anastomotic Leak/diagnosis [MeSH] ; Original Article ; Chemokine CCL8 [MeSH] ; Anastomotic Leak/etiology [MeSH] ; Early Detection of Cancer [MeSH] ; Anastomosis, Surgical [MeSH] ; Anastomotic leakage ; Biomarkers [MeSH]; Biomarkers; Early Detection of Cancer

Abstract Purpose Anastomotic leakage constitutes a dreaded complication after colorectal surgery, leading to increased morbidity and mortality as well as prolonged hospitalization. Most leakages become clinically apparent about 8 days after surgery; however, early detection is quintessential to reduce complications and to improve patients’ outcome. We therefore investigated the significance of specific protein expression profiles as putative biomarkers, indicating anastomotic leakage.

Methods In this single-center prospective cohort study serum and peritoneal fluid samples—from routinely intraoperatively inserted drainages—of colorectal cancer patients were collected 3 days after colorectal resection. Twenty patients without anastomotic leakage and 18 patients with an anastomotic leakage and without other complications were included. Protein expression of seven inflammatory markers in serum and peritoneal fluid was assessed by multiplex ELISA and correlated with patients’ clinical data.

Results Monocyte chemoattractant protein 2 (CCL8/MCP-2), leukemia-inhibiting factor (LIF), and epithelial-derived neutrophil-activating protein (CXCL5/ENA-78) were significantly elevated in peritoneal fluid but not in serum samples from patients subsequently developing anastomotic leakage after colorectal surgery. No expressional differences could be found between grade B and grade C anastomotic leakages.

Conclusion Measurement 3 days after surgery revealed altered protein expression patterns of the inflammatory markers CCL8/MCP2, LIF, and CXCL5/ENA-78 in peritoneal fluid from patients developing anastomotic leakage after colorectal surgery. Further studies with a larger patient cohort with inclusion of different variables are needed to evaluate their potential as predictive biomarkers for anastomotic leakage.