Moderate physical training attenuates perinatal low-protein-induced spleen lymphocyte apoptosis in endotoxemic adult offspring rats

• DOI: 10.1007/s00394-015-0925-y
• Publication Date: 2015-05-18T07:29:22Z
• Authors: Sueli Moreno Senna; Marília Kalinne T...; Daíllo Augusto Pe...; Maria Claudia Alh...; Diógenis Barbosa ...; Valéria Rêgo Alve...; Francisco Carlos ...; José Candido Ferraz; Carol Góis Leandro
• Keywords: Male; 2. Zero hunger; 0303 health sciences; Tumor Necrosis Factor-alpha; Malnutrition; Apoptosis; DNA Fragmentation; Maternal Nutritional Physiological Phenomena; Phosphatidylserines; Endotoxemia; Lymphocyte Subsets; Rats; Perinatal Care; 03 medical and health sciences; Pregnancy; Physical Conditioning, Animal; Prenatal Exposure Delayed Effects; Diet, Protein-Restricted; Animals; Female; Dietary Proteins; Rats, Wistar; Spleen

To evaluate the effects of a moderate physical training (T) on the blood and splenic lymphocytes subsets and the rate of apoptosis in adult offspring submitted to perinatal low-protein (LP) diet.Male Wistar rats were divided according to their mother's diet: control (C, 17 % casein) and undernourished (LP, 8 % casein). At the 60th day, pups were submitted to moderate physical training (8 weeks, 5 days week(-1), 60 min day(-1), at 70 % of VO2max). After T period, pups received an injection of lipopolysaccharide (LPS). B, NK, and TCD3+ lymphocytes subsets were analyzed by flow cytometry. Spleen lymphocytes apoptosis was evaluated by DNA fragmentation, phosphatidylserine externalization (PSE), and mitochondrial transmembrane depolarization (MTD) using a flow cytometer. Plasma TNF-α concentrations were analyzed by ELISA.LP + LPS pups showed a higher percentage of blood B, CD4+, and NK and a reduction in TCD3+, CD8+ than C pups. The percentage of NK and CD3+ was restored in LP + T + LPS pups. In the spleen, T normalized the percentage of NK in LP + LPS pups. LP + LPS pups showed a higher percentage of cells with PSE and MTD than C + LPS pups that was attenuated by T. The concentration of TNF-α was higher in LP + LPS than C + LPS, but it was attenuated in LP + T + LPS pups.Moderate physical training was able to revert the effects of perinatal LP diet on circulation lymphocytes subsets and attenuated splenic lymphocytes apoptosis and plasma TNF-α concentrations.