Inulin supplementation ameliorates hyperuricemia and modulates gut microbiota in Uox-knockout mice

• DOI: 10.1007/s00394-020-02414-x
• Publication Date: 2020-10-26T12:03:10Z
• Authors: Yingjie Guo; Yanan Yu; Hailong Li; Xueli Ding; Xiaoyu Li; Xue Jing; Jianwei Chen; Guilin Liu; Yuan Lin; Chen Jiang; Zhen Liu; Yuwei He; Changgui Li; Zibin Tian
• Keywords: Akkermansia muciniphila; Akkermansia

Abstract Purpose Inulin is a type of fermentable dietary fiber, which non-digestible, and can improve metabolic function by modulating intestinal microbiota. This study aimed to evaluate the role inulin in hyperuricemia microbial composition gut microbiota mouse model established through knockout Uox (urate oxidase) gene. Methods KO ( -knockout) WT (wild-type) mice were given or saline gavage for 7 weeks. The effect combat was determined assessing changes serum UA (uric acid) levels, inflammatory parameters, epithelial barrier integrity, fecal alterations, SCFA (short-chain fatty concentrations mice. Results supplementation effectively alleviate hyperuricemia, increase expressions ABCG2 intestine, downregulate expression activity hepatic XOD (xanthine It revealed that levels cytokines LPS (lipopolysaccharide) remarkably higher group than those group, indicating systemic inflammation hyperuricemic mice, but treatment ameliorated Besides, repaired as evidenced increased TJ (tight junction) proteins [ZO-1 (zonula occludens-1) occluding] Moreover, uremic toxins, including IS (indoxyl sulfate) PCS p -cresol sulfate), reduced inulin-treated Further investigation unveiled enhanced diversity raised relative abundance beneficial bacteria, involving SCFAs-producing bacteria (e.g., Akkermansia Ruminococcus ). Additionally, production microbiota-derived SCFAs (acetate, propionate butyrate concentrations) positively correlated with effectiveness relief. Conclusions Our findings showed may be promising therapeutic candidate hyperuricemia. alleviation was, at least, partially conciliated modulation its metabolites.