Developing a mouse model of acute bacterial rhinosinusitis

• DOI: 10.1007/s00405-011-1483-4
• Publication Date: 2011-01-25T13:04:49Z
• Authors: Mingzhu Jin; Zhaowei Gu; Zhigang Bian; Jing Yang; Zhiwei Cao; Xiaofeng Yu; Genyan Guo
• Keywords: Male; Methicillin-Resistant Staphylococcus aureus; Mice, Inbred BALB C; Staphylococcal Infections; Nasal Lavage Fluid; Severity of Illness Index; 3. Good health; Disease Models, Animal; Mice; Nasal Mucosa; 03 medical and health sciences; 0302 clinical medicine; Acute Disease; Animals; Sinusitis; Rhinitis

The objective of this study is to establish a mouse model of acute bacterial rhinosinusitis. 179 healthy male BALB/c mice were divided into four groups in this randomized and controlled study. Sponge slivers impregnated with methicillin-resistant Staphylococcus aureus (MRSA COL) suspension were inserted into the right nasal cavities for group A; sponge slivers impregnated with sterile saline were inserted into the right nasal cavities for group B; group C mice were inoculated with MRSA COL suspension in right nasal cavities; group D was control group without any treatment. Mice were killed on days 1, 4, 7 and 14, respectively. Nasal lavage fluid was prepared for microbiological culture. Histological examinations of nasal specimens were performed to observe the severity of inflammatory reaction. Acute bacterial rhinosinusitis was induced in all group A mice. Less severe inflammation was seen in partial group B mice compared with that in group A mice (P ≤ 0.05). No inflammatory reaction was found in group C and D mice. In conclusion, a mouse model of acute bacterial rhinosinusitis has been developed successfully using an easier, less invasive and potentially more reversible technique than those used in previous studies.