Toward the development of transcriptional biodosimetry for the identification of irradiated individuals and assessment of absorbed radiation dose

• DOI: 10.1007/s00411-015-0603-8
• Publication Date: 2015-05-13T12:30:53Z
• Authors: Kamil Brzóska; Marcin Kruszewski
• Keywords: Biodosimetry; Radioresistance

The most frequently used and the best established method of biological dosimetry at present is dicentric chromosome assay, which poorly suitable for a mass casualties scenario. This gives rise to need development new, high-throughput assays rapid identification subjects exposed ionizing radiation. In study, we tested usefulness gene expression analysis in blood cells dosimetry. Human peripheral from three healthy donors was X-irradiated with doses 0 (control), 0.6, 2 Gy. mRNA level 16 genes (ATF3, BAX, BBC3, BCL2, CDKN1A, DDB2, FDXR, GADD45A, GDF15, MDM2, PLK3, SERPINE1, SESN2, TNFRSF10B, TNFSF4, VWCE) assessed by reverse transcription quantitative PCR 6, 12, 24, 48 h after exposure ITFG1 DPM1 as reference genes. panel radiation-responsive selected comprising FDXR. Cluster showed that ΔC t values contained sufficient information allow discrimination between irradiated non-irradiated samples. samples were clearly grouped according absorbed radiation not time interval irradiation or donor.