Mutability and mutational spectrum of chromosome transmission fidelity genes

• DOI: 10.1007/s00412-011-0356-3
• Publication Date: 2011-12-23T13:25:58Z
• Authors: Peter C. Stirling; Matthew J. Crisp; Munira A. Basrai; Cheryl M. Tucker; Maitreya J. Dunham; Forrest A. Spencer; Philip Hieter
• Keywords: Genetic screen

It has been more than two decades since the original chromosome transmission fidelity (Ctf) screen of Saccharomyces cerevisiae was published. Since that time spectrum mutations known to cause Ctf and, generally, instability (CIN) expanded dramatically as a result systematic screens across yeast mutant arrays. Here we describe comprehensive summary genetic and cloning remaining complementation groups efforts expand our knowledge CIN gene repertoire its mutability in model eukaryote. At screen, it impossible predict either genes processes would be overrepresented pool random mutants displaying phenotype or what entire set potentially mutable be. We show collection 136 randomly selected mutants, >65% map 13 genes, 12 which are involved sister chromatid cohesion and/or kinetochore function. Extensive screening collections shown ~350 with functions diverse RNA processing proteasomal activity mutate at least 692 required for faithful segregation. The enrichment alleles only possible suggests these easily genome others. These observations inform understanding recurring human cancers where presumably responsible initiating frequently observed tumors.