Effect of gravity in long-term vitreous tamponade: in vivo investigation using perfluorocarbon liquids and semi-fluorinated alkanes

• DOI: 10.1007/s00417-006-0414-3
• Publication Date: 2006-10-10T10:21:52Z
• Authors: Jerzy Mackiewicz; Kristel Maaijwee; Christoph Lüke; Norbert Kociok; Wilfried Hiebl; Hasso Meinert; Antonia M. Joussen
• Keywords: Fluorocarbons; Retinal Degeneration; Cell Count; Retina; Vitreous Body; Drug Combinations; 03 medical and health sciences; 0302 clinical medicine; Electroretinography; In Situ Nick-End Labeling; Animals; Rabbits; Specific Gravity; Photic Stimulation; Photoreceptor Cells, Vertebrate

In order to investigate whether gravity is the reason for retinal degeneration in long-term vitreous tamponade, perfluorohexyloctane (F6H8), perfluorodecalin (PFD), and a mixture of F6H8/PFD were compared.Each group of 5 rabbits received a 3-month tamponade with either PFD (pure) (1.93 g/cm(3)), F6H8 (pure) (1.33 g/cm(3)), or a 1:1 mixture of F6H8/PFD (1.62 g/cm(3)). Electroretinograms (ERG) were performed pre- and postoperatively. Lower and upper retinal areas were compared using immunohistochemical methods. Transmission electron microscopy was performed to investigate alterations in the photoreceptors.All three substances were tolerated well in rabbit eyes for up to 3 months. Dispersion was seen earliest with PFD and latest with pure F6H8. None of the substances demonstrated inflammatory reactions or vascular alterations. ERGs were not considerably altered with any of the substances. Histology of the retina showed alterations in the cell counts within the inner and outer nuclear layer that were not attributable to the gravity of the tamponading agent.In contrast to previously published work, this study did not detect any tamponade-related structural damage of the retina after a 3-months tamponade in the rabbit model. Based upon this study, we conclude that gravity might not be causally linked to retinal damage.