Monocytes subsets altered distribution and dysregulated plasma hsa-miR-21-5p and hsa-miR-155-5p in HCV-linked liver cirrhosis progression to hepatocellular carcinoma

• DOI: 10.1007/s00432-023-05313-w
• Publication Date: 2023-08-28T11:03:31Z
• Authors: Reham Hammad; Mona A. Eldosoky; Asmaa A. Elmadbouly; Reda Badr Aglan; Sherihan G. Abdel...; Samy Zaky; Elham Ali; Fatma El-Zahraa A...; Alshaimaa M. Mosaad; Neamat A. Abdelma...; Fatma M. Kotb; Hend G. Kotb; Ahmed A. Hady; Omaima I. Abo-Elk...; Sandy Kujumdshiev; Ulrich Sack; Claude Lambert; Nadia M. Hamdy
• Keywords: Hematology

Abstract Purpose The authors aim to investigate the altered monocytes subsets distribution in liver cirrhosis (LC) and subsequent hepatocellular carcinoma (HCC) association with expression level of plasma Homo sapiens (has)-miR-21-5p hsa-miR-155-5p. A step toward non-protein coding (nc) RNA precision medicine based on immune perturbation manifested as distribution, top LC HCC. Methods Seventy-nine patients diagnosed chronic hepatitis C virus (CHCV) infection were enrolled current study. Patients sub-classified into group without HCC ( n = 40), 39), 15 apparently healthy controls. Monocyte frequencies assessed by flow cytometry. Real-time quantitative PCR was used measure hsa-miR-21-5p hsa-miR-155-5p expression. Results Hsa-miR-21-5p correlated intermediate r 0.30, p 0.007), while negatively non-classical − 0.316, 0.005). ROC curve analysis revealed that combining frequency hsa-miR-21 yielded sensitivity 79.5%, specificity 75%, AUC 0.84. In comparison, AFP a lower 69% 100% 0.85. Logistic regression proved up-regulation independent risk factors for progression HCC, after adjustment co-founders. Conclusion differentiation linked Combined could be considered sensitive indicator Circulating evolution, clinically silico proved. Graphical abstract