Dominant infection of Cryptosporidium baileyi in broiler chickens in Zhejiang Province, China

• DOI: 10.1007/s00436-023-07898-0
• Publication Date: 2023-06-22T06:05:34Z
• Authors: Xinwei Feng; Jinhua Deng; Zhenjie Zhang; Fuchang Yu; Jianing Zhang; Tuanyuan Shi; Hongchao Sun; Meng Qi; Xuehan Liu
• Keywords: China; Feces; Genotype; Humans; Animals; Cryptosporidium; Cryptosporidiosis; Chickens; Poultry Diseases

Cryptosporidium is a common enteric parasite in chickens. A total of 812 fecal specimens were collected from 11 broiler farms in Zhejiang Province, China, and analyzed by nested PCR amplification based on the small subunit ribosomal RNA (SSU rRNA) gene. The overall infection rate of Cryptosporidium was 6.3% (51/812), and five of 11 farms were Cryptosporidium positive. Broilers aged > 90 days accounted for the highest infection rate of 16.1% (6/56), followed by those aged 30-60 days (10.6%, 38/358) and 60-90 days (4/378, 1.1%). Two Cryptosporidium species were identified by sequence analysis, with the predominant species being C. baileyi (96.1%, 49/51) and the minor infection being C. meleagridis (3.9%, 2/51). Based on the 60-kDa glycoprotein (gp60) gene, two C. meleagridis-positive isolates were identified as one known subtype, IIIbA24G1R1. This study indicated the common occurrence of C. baileyi in broiler chickens in this region and low zoonotic transmission potential of Cryptosporidium to humans.